Difference between revisions of "Gill:TSB (Tryptone Soy Broth)"

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==Notes==
 
==Notes==
 
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
 
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
 +
 +
* Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass.
  
 
[[Category:Protocol]]
 
[[Category:Protocol]]

Latest revision as of 07:29, 1 May 2013

Materials

500 mL:

  • 15 g TSB (Bacto)
  • 500 ml dH2O

1000 mL:

  • 30 g TSB (Bacto)
  • 1000 ml dH2O

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  • Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass.