Difference between revisions of "Gill:TNB (Tryptone Nutrient Broth)"

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(New page: ==Materials== 500 mL: *Tryptone 2.5 g *Yeast extract 1.25 g *Glucose 0.5 g *NaCl 4.25 g *dH<sub>2</sub>O 500 ml ==Procedure== Mix all ingredients in a 1 litre bottle or flask,...)
 
 
Line 3: Line 3:
 
500 mL:
 
500 mL:
  
*Tryptone 2.5 g
+
*2.5 g Tryptone
*Yeast extract 1.25 g
+
*1.25 g Yeast extract
*Glucose 0.5 g
+
*0.5 g Glucose
*NaCl 4.25 g
+
*4.25 g NaCl
*dH<sub>2</sub>O 500 ml
+
*500 ml dH<sub>2</sub>O
  
 
==Procedure==
 
==Procedure==

Latest revision as of 13:56, 23 April 2013

Materials

500 mL:

  • 2.5 g Tryptone
  • 1.25 g Yeast extract
  • 0.5 g Glucose
  • 4.25 g NaCl
  • 500 ml dH2O

Procedure

Mix all ingredients in a 1 litre bottle or flask, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!