Difference between revisions of "Gill:TNA (Tryptone Nutrient Agar)"

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(New page: ==Materials== 500 mL: *Tryptone 2.5 g *Yeast extract 1.25 g *Glucose 0.5 g *NaCl 4.25 g *Agar 7.5 g *dH<sub>2</sub>O 500 ml ==Procedure== Mix all ingredients in a 1 litre bott...)
 
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500 mL:
 
500 mL:
  
*Tryptone 2.5 g
+
*2.5 g Tryptone
*Yeast extract 1.25 g
+
*1.25 g Yeast extract
*Glucose 0.5 g
+
*0.5 g Glucose
*NaCl 4.25 g
+
*4.25 g NaCl
*Agar 7.5 g
+
*7.5 g Agar
*dH<sub>2</sub>O 500 ml
+
*500 ml dH<sub>2</sub>O
  
 
==Procedure==
 
==Procedure==

Latest revision as of 12:55, 23 April 2013

Materials

500 mL:

  • 2.5 g Tryptone
  • 1.25 g Yeast extract
  • 0.5 g Glucose
  • 4.25 g NaCl
  • 7.5 g Agar
  • 500 ml dH2O

Procedure

Mix all ingredients in a 1 litre bottle or flask, cover and autoclave. Label caps with “TNA” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!