Gill:Supplemented M9 broth (M9sup)
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Revision as of 12:34, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: ==Materials== {| class="wikitable" |- ! ! 50 mL ! 500 mL ! 1000 mL |- | dH<sub>2</sub>O | 43 ml | 430 ml | 860 ml |- | 10 X M9 salts + glucose | 5 ml | 50 ml | 100 ml |- | 10% Casamino...)
Materials
50 mL | 500 mL | 1000 mL | |
---|---|---|---|
dH_{2}O | 43 ml | 430 ml | 860 ml |
10 X M9 salts + glucose | 5 ml | 50 ml | 100 ml |
10% Casamino acids | 1 ml | 10 ml | 20 ml |
1% Thiamine | 50 µl | 0.5 ml | 1 ml |
1 M MgSO_{4} | 100 µl | 1 ml | 2 ml |
1 M CaCl_{2} | 5 µl | 50 µl | 0.1 ml |
Procedure
Pre-sterilize all component solutions and aseptically add to sterile water. Alternatively, mix all components and then filter-sterilize through a 0.22 µm filter. Store at 4 ºC.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!