Difference between revisions of "Gill:Supplemented M9 broth (M9sup)"
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(New page: ==Materials== { class="wikitable"  ! ! 50 mL ! 500 mL ! 1000 mL   dH<sub>2</sub>O  43 ml  430 ml  860 ml   10 X M9 salts + glucose  5 ml  50 ml  100 ml   10% Casamino...) 
(No difference)

Latest revision as of 12:34, 23 April 2013
Materials
50 mL  500 mL  1000 mL  

dH_{2}O  43 ml  430 ml  860 ml 
10 X M9 salts + glucose  5 ml  50 ml  100 ml 
10% Casamino acids  1 ml  10 ml  20 ml 
1% Thiamine  50 µl  0.5 ml  1 ml 
1 M MgSO_{4}  100 µl  1 ml  2 ml 
1 M CaCl_{2}  5 µl  50 µl  0.1 ml 
Procedure
Presterilize all component solutions and aseptically add to sterile water. Alternatively, mix all components and then filtersterilize through a 0.22 µm filter. Store at 4 ºC.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!