Difference between revisions of "Gill:Protocols"

From OpenWetWare
Jump to: navigation, search
Line 12: Line 12:
[[Gill:Making plate lysates |Making plate lysates]]
[[Gill:Making plate lysates |Making plate lysates]]
[[Gill:Phage enrichment |Phage enrichment]]
[[Gill:Phage genomic DNA extraction |Phage genomic DNA extraction]]
[[Gill:Phage genomic DNA extraction |Phage genomic DNA extraction]]

Revision as of 15:11, 11 November 2013

Gill header.jpg

Home        Research        Protocols        Lab Members        Publications        Internal        Contact       


Microbiology and Molecular Biology

Chung cells

Confirming phage via gel electrophoresis

Making frozen permanents

Making plate lysates

Phage enrichment

Phage genomic DNA extraction

S. aureus genomic DNA isolation

Subculturing phage isolates

Solutions and Media Preparation

Lambda diluent (SM buffer)

10x Lambda diluent (no gelatin)

LB agar

LB broth

M9 agar

Supplemented M9 agar (M9sup)

Supplemented M9 broth (M9sup)

10 X M9 salts

10 X M9 salts + glucose

S liquid medium

TNA (Tryptone Nutrient Agar)


TNB (Tryptone Nutrient Broth)


TSA (Tryptone Soy Agar)

TSB (Tryptone Soy Broth)

T-top (0.5% agar)

Vogel-Bonner glucose (VBG) minimal medium

50X Vogel-Bonner salts