Difference between revisions of "Gill:Protocols"

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[[Gill:Making frozen permanents |Making frozen permanents]]
[[Gill:Making frozen permanents |Making frozen permanents]] <br>
[[Gill:Confirming phage via gel electrophoresis |Confirming phage via gel electrophoresis]]
===Solutions and Media Preparation===
===Solutions and Media Preparation===

Revision as of 09:43, 25 June 2013

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Making frozen permanents

Confirming phage via gel electrophoresis

Solutions and Media Preparation

TNA (Tryptone Nutrient Agar)

TNB (Tryptone Nutrient Broth)



Lambda diluent (SM buffer)

10x Lambda diluent (no gelatin)

TSB (Tryptone Soy Broth)

TSA (Tryptone Soy Agar)

LB broth

LB agar

S liquid medium

T-top (0.5% agar)

10 X M9 salts

10 X M9 salts + glucose

M9 agar

Supplemented M9 agar (M9sup)

Supplemented M9 broth (M9sup)

Vogel-Bonner glucose (VBG) minimal medium

50X Vogel-Bonner salts