Gill:M9 agar
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Materials
500 mL:
- Bacto agar 7.5 g
- dH2O 450 ml
- 10 X M9 salts + glucose 50 ml
- 1 M MgSO4 1 ml (2 mM final)
- 1 M CaCl2 50 µl (0.1 mM final)
1000 mL:
- Bacto agar 15 g
- dH2O 880 ml
- 10 X M9 salts + glucose 100 ml
- 1 M MgSO4 2 ml (2 mM final)
- 1 M CaCl2 0.1 ml (0.1 mM final)
Procedure
Autoclave agar/water solution, let cool. Aseptically add sterile salts + glucose, pour.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!