Gill:Lambda diluent (SM buffer)

From OpenWetWare
Revision as of 11:53, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: ==Materials== 1000 mL: * 1M Tris-HCl, pH 7.5, 50 ml (25 mM) * NaCl 5.8 g (100 mM) * MgSO4 1 g (8 mM) * Gelatin (2% sol’n) 5 ml (0.01 %) * dH<sub>2</sub>O 475 ml ==Procedure== Mix all ...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Materials

1000 mL:

  • 1M Tris-HCl, pH 7.5, 50 ml (25 mM)
  • NaCl 5.8 g (100 mM)
  • MgSO4 1 g (8 mM)
  • Gelatin (2% sol’n) 5 ml (0.01 %)
  • dH2O 475 ml

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!