Difference between revisions of "Gill:Lambda diluent (SM buffer)"

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==Materials==
 
==Materials==
 
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{| class="wikitable"
1000 mL:
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|-
 
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!
* 50 ml (25 mM) 1M Tris-HCl, pH 7.5
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! 1000 mL
* 5.8 g (100 mM) NaCl
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|-
* 1 g (8 mM) MgSO4
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|1M Tris-HCl, pH 7.5
* 5 ml (0.01 %) Gelatin (2% sol’n)
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|50 ml (25 mM)
* 475 ml dH<sub>2</sub>O
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|-
 +
|NaCl
 +
|5.8 g (100 mM)
 +
|-
 +
|MgSO<sub>4</sub>
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|1 g (8 mM)
 +
|-
 +
|Gelatin (2% sol’n)
 +
|5 ml (0.01 %)
 +
|-
 +
|dH<sub>2</sub>O
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|475 ml
 +
|}
  
 
==Procedure==
 
==Procedure==

Latest revision as of 14:02, 23 April 2013

Materials

1000 mL
1M Tris-HCl, pH 7.5 50 ml (25 mM)
NaCl 5.8 g (100 mM)
MgSO4 1 g (8 mM)
Gelatin (2% sol’n) 5 ml (0.01 %)
dH2O 475 ml

Procedure

Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!