Gill:LB broth

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Revision as of 13:00, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: ==Materials== 1000 mL: *Bacto tryptone 10 g *NaCl 10 g *Yeast extract 5 g *dH<sub>2</sub>O 1000 ml ==Procedure== Dissolve medium in water, then aliquot 100 ml into square milk bottles...)
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Materials

1000 mL:

  • Bacto tryptone 10 g
  • NaCl 10 g
  • Yeast extract 5 g
  • dH2O 1000 ml

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!