Difference between revisions of "Gill:LB broth"

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(New page: ==Materials== 1000 mL: *Bacto tryptone 10 g *NaCl 10 g *Yeast extract 5 g *dH<sub>2</sub>O 1000 ml ==Procedure== Dissolve medium in water, then aliquot 100 ml into square milk bottles...)
 
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
1000 mL:
 
1000 mL:
  
*Bacto tryptone 10 g
+
*10 g Bacto tryptone
*NaCl 10 g
+
*10 g NaCl (5 g NaCl for LB Lennox)
*Yeast extract 5 g
+
*5 g Yeast extract
*dH<sub>2</sub>O 1000 ml
+
*1000 ml dH<sub>2</sub>O
  
 
==Procedure==
 
==Procedure==

Latest revision as of 13:23, 7 November 2013

Materials

1000 mL:

  • 10 g Bacto tryptone
  • 10 g NaCl (5 g NaCl for LB Lennox)
  • 5 g Yeast extract
  • 1000 ml dH2O

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!