Gill:LB agar

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Revision as of 12:01, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: ==Materials== 1000 mL: *Bacto tryptone 10 g *NaCl 10 g *Yeast extract 5 g *dH<sub>2</sub>O 1000 ml *Bacto agar 7.5 g ==Procedure== Dissolve medium in water. Add 7.5 g Bacto agar to e...)
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Materials

1000 mL:

  • Bacto tryptone 10 g
  • NaCl 10 g
  • Yeast extract 5 g
  • dH2O 1000 ml
  • Bacto agar 7.5 g

Procedure

Dissolve medium in water. Add 7.5 g Bacto agar to empty 1 L bottles, aliquot 500 ml of medium to each bottle. Label caps with “LB” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!