Gill:50X Vogel-Bonner salts: Difference between revisions
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(New page: ==Materials== {| class="wikitable" |- ! ! 1000 mL |- |Warm dd H2O (45°C) |670 ml |- |MgSO4 • 7 H2O |10 g |- |Citric acid monohydrate |100 g |- |K2HPO4 |500 g |- ...) |
mNo edit summary |
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| Line 6: | Line 6: | ||
! 1000 mL | ! 1000 mL | ||
|- | |- | ||
|Warm dd | |Warm dd H<sub>2</sub>O (45°C) | ||
|670 ml | |670 ml | ||
|- | |- | ||
| | |MgSO<sub>4</sub> • 7 H<sub>2</sub>O | ||
|10 g | |10 g | ||
|- | |- | ||
| Line 15: | Line 15: | ||
|100 g | |100 g | ||
|- | |- | ||
| | |K<sub>2</sub>HPO<sub>4</sub> | ||
|500 g | |500 g | ||
|- | |- | ||
| | |NaHNH<sub>4</sub>PO<sub>4</sub> • 4H<sub>2</sub>O | ||
|175 g | |175 g | ||
|} | |} | ||
Latest revision as of 13:42, 23 April 2013
Materials
| 1000 mL | |
|---|---|
| Warm dd H2O (45°C) | 670 ml |
| MgSO4 • 7 H2O | 10 g |
| Citric acid monohydrate | 100 g |
| K2HPO4 | 500 g |
| NaHNH4PO4 • 4H2O | 175 g |
Procedure
Add salts in the order indicated to warm water in a 2-liter flask placed on magnetic stirring hot plate. Allow each salt to dissolve completely before adding the next. Adjust the volume to 1 liter. Distribute into two 1-liter glass bottles. Autoclave for 20 min at 121°C, store at RT.
Notes
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
