Difference between revisions of "Gill:50X Vogel-Bonner salts"

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(New page: ==Materials== {| class="wikitable" |- ! ! 1000 mL |- |Warm dd H2O (45°C) |670 ml |- |MgSO4 • 7 H2O |10 g |- |Citric acid monohydrate |100 g |- |K2HPO4 |500 g |- ...)
 
m
 
Line 6: Line 6:
 
! 1000 mL
 
! 1000 mL
 
|-
 
|-
|Warm dd H2O (45°C)
+
|Warm dd H<sub>2</sub>O (45°C)
 
|670 ml
 
|670 ml
 
|-
 
|-
|MgSO4 • 7 H2O
+
|MgSO<sub>4</sub> • 7 H<sub>2</sub>O
 
|10 g
 
|10 g
 
|-
 
|-
Line 15: Line 15:
 
|100 g  
 
|100 g  
 
|-
 
|-
|K2HPO4
+
|K<sub>2</sub>HPO<sub>4</sub>
 
|500 g     
 
|500 g     
 
|-  
 
|-  
|NaHNH4PO4 4H2O
+
|NaHNH<sub>4</sub>PO<sub>4</sub> 4H<sub>2</sub>O
 
|175 g     
 
|175 g     
 
|}
 
|}

Latest revision as of 12:42, 23 April 2013

Materials

1000 mL
Warm dd H2O (45°C) 670 ml
MgSO4 • 7 H2O 10 g
Citric acid monohydrate 100 g
K2HPO4 500 g
NaHNH4PO4 • 4H2O 175 g

Procedure

Add salts in the order indicated to warm water in a 2-liter flask placed on magnetic stirring hot plate. Allow each salt to dissolve completely before adding the next. Adjust the volume to 1 liter. Distribute into two 1-liter glass bottles. Autoclave for 20 min at 121°C, store at RT.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!