Gill:10x Lambda diluent (no gelatin)
Revision as of 12:55, 23 April 2013 by Jacqueline K. Grimm (New page: ==Materials== 1000 mL: *Tris base 60.56 g *NaCl 58.0 g *MgSO4 10 g *dH<sub>2</sub>O to 1000 ml ==Procedure== Dissolve in 800 ml dH<sub>2</sub>O, adjust pH to 7.5 with HCl, add water to ...)
- Tris base 60.56 g
- NaCl 58.0 g
- MgSO4 10 g
- dH2O to 1000 ml
Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Autoclave, store at room temp.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!