Difference between revisions of "Gill:10x Lambda diluent (no gelatin)"

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(New page: ==Materials== 1000 mL: *Tris base 60.56 g *NaCl 58.0 g *MgSO4 10 g *dH<sub>2</sub>O to 1000 ml ==Procedure== Dissolve in 800 ml dH<sub>2</sub>O, adjust pH to 7.5 with HCl, add water to ...)
 
m
 
Line 3: Line 3:
 
1000 mL:
 
1000 mL:
  
*Tris base 60.56 g
+
*60.56 g Tris base
*NaCl 58.0 g
+
*58.0 g NaCl
*MgSO4 10 g
+
*10 g MgSO4
 
*dH<sub>2</sub>O to 1000 ml
 
*dH<sub>2</sub>O to 1000 ml
  

Latest revision as of 13:04, 23 April 2013

Materials

1000 mL:

  • 60.56 g Tris base
  • 58.0 g NaCl
  • 10 g MgSO4
  • dH2O to 1000 ml

Procedure

Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Autoclave, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!