Gill:10 X M9 salts + glucose

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Revision as of 12:14, 23 April 2013 by Jacqueline K. Grimm (talk | contribs) (New page: ==Materials== 1000 mL: *dH<sub>2</sub>O 700 ml *Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g *KH<sub>2</sub>PO<sub>4</sub> 30 g *NaCl 5 g *NH<sub>4</sub>Cl 10 g *Glucose 40 g ...)
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Materials

1000 mL:

  • dH2O 700 ml
  • Na2HPO4 • 7H2O 128 g
  • KH2PO4 30 g
  • NaCl 5 g
  • NH4Cl 10 g
  • Glucose 40 g

Procedure

Add all components to water, dissolve, adjust to 1000 ml. Filter sterilize at 0.22 µm (do not autoclave), store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!