Difference between revisions of "Gill:10 X M9 salts"

From OpenWetWare
Jump to: navigation, search
(New page: ==Materials== 1000 mL: *dH<sub>2</sub>O 700 ml *Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g *KH<sub>2</sub>PO<sub>4</sub> 30 g *NaCl 5 g *NH<sub>4</sub>Cl 10 g ==Procedure==...)
(No difference)

Revision as of 12:13, 23 April 2013

Materials

1000 mL:

  • dH2O 700 ml
  • Na2HPO4 • 7H2O 128 g
  • KH2PO4 30 g
  • NaCl 5 g
  • NH4Cl 10 g

Procedure

Add salts to water, dissolve, adjust to 1000 ml. Autoclave, store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!