Difference between revisions of "Gill:10 X M9 salts"

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(New page: ==Materials== 1000 mL: *dH<sub>2</sub>O 700 ml *Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g *KH<sub>2</sub>PO<sub>4</sub> 30 g *NaCl 5 g *NH<sub>4</sub>Cl 10 g ==Procedure==...)
 
m
 
Line 3: Line 3:
 
1000 mL:
 
1000 mL:
  
*dH<sub>2</sub>O 700 ml
+
*700 ml dH<sub>2</sub>O
*Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O 128 g
+
*128 g Na<sub>2</sub>HPO<sub>4</sub> • 7H<sub>2</sub>O
*KH<sub>2</sub>PO<sub>4</sub> 30 g
+
*30 g KH<sub>2</sub>PO<sub>4</sub>
*NaCl 5 g
+
*5 g NaCl
*NH<sub>4</sub>Cl 10 g
+
*10 g NH<sub>4</sub>Cl
  
 
==Procedure==
 
==Procedure==

Latest revision as of 13:10, 23 April 2013

Materials

1000 mL:

  • 700 ml dH2O
  • 128 g Na2HPO4 • 7H2O
  • 30 g KH2PO4
  • 5 g NaCl
  • 10 g NH4Cl

Procedure

Add salts to water, dissolve, adjust to 1000 ml. Autoclave, store at RT. If using anhydrous Na2HPO4, multiply the weight for this component by 0.53.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!