Difference between revisions of "Gentamicin"

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===Resistance Gene===
 
===Resistance Gene===
The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001.  It conveys resistance to both Gentamycin and Kanamycin in two separate domains.  Another common resistance gene is the aacC1 gene, (conveying resistance to Gentamycin only) is found in the plasmid pJN105.  <cite>Newman-Gene-1999, Wohlleben-MolGenGenet-1989</cite>
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The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001.  It conveys resistance to both Gentamicin and Kanamycin in two separate domains.  Another common resistance gene is the aacC1 gene, (conveying resistance to Gentamicin only) is found in the plasmid pJN105.  <cite>Newman-Gene-1999, Wohlleben-MolGenGenet-1989</cite>
  
 
===Safety===
 
===Safety===
Gentamycin is an important antibiotic for human use.  Think about alternative antibiotics when choosing to incorporate gentamycin resistance into your constructs.  Particularly, think carefully about inserting gentamycin resistance into mobile plasmids or mobile transposons.
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Gentamicin is an important antibiotic for human use.  Think about alternative antibiotics when choosing to incorporate gentamicin resistance into your constructs.  Particularly, think carefully about inserting gentamicin resistance into mobile plasmids or mobile transposons.
  
 
==References==
 
==References==
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#Wohlleben-MolGenGenet-1989 pmid=2549372
 
#Wohlleben-MolGenGenet-1989 pmid=2549372
 
</biblio>
 
</biblio>
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[[Category:Material]] [[Category:Antibiotic]]

Latest revision as of 11:33, 22 July 2008

Experimental

Stock Solution

10mg/ml in H20, store at -20°C (from here)

Soluble in water at least to 200 mg/ml

Insoluble in 50% alcohol

Working Concentration

20μg/ml [1]

80μg/ml for Mycoplasma selection

Mode of Action

Inhibits protein synthesis by binding to L6 protein of the 50S ribosomal subunit.

(from Molecular Cloning)

Resistance Gene

The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001. It conveys resistance to both Gentamicin and Kanamycin in two separate domains. Another common resistance gene is the aacC1 gene, (conveying resistance to Gentamicin only) is found in the plasmid pJN105. [2, 3]

Safety

Gentamicin is an important antibiotic for human use. Think about alternative antibiotics when choosing to incorporate gentamicin resistance into your constructs. Particularly, think carefully about inserting gentamicin resistance into mobile plasmids or mobile transposons.

References

  1. Khlebnikov A, Skaug T, and Keasling JD. Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7. DOI:10.1038/sj.jim.7000259 | PubMed ID:12080425 | HubMed [Khlebnikov-JIndMicrobiolBiotechnol-2002]
  2. Newman JR and Fuqua C. Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator. Gene. 1999 Feb 18;227(2):197-203. PubMed ID:10023058 | HubMed [Newman-Gene-1999]
  3. Wohlleben W, Arnold W, Bissonnette L, Pelletier A, Tanguay A, Roy PH, Gamboa GC, Barry GF, Aubert E, and Davies J. On the evolution of Tn21-like multiresistance transposons: sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette. Mol Gen Genet. 1989 Jun;217(2-3):202-8. PubMed ID:2549372 | HubMed [Wohlleben-MolGenGenet-1989]
All Medline abstracts: PubMed | HubMed