GYT

From OpenWetWare
Revision as of 09:53, 22 July 2008 by Andrew Ang (talk | contribs) (Ingredients)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Summary

GYT Medium. Used in preparing electrocompetent cells.

Ingredients

  • 10% (v/v) glycerol
  • 0.125% (w/v) yeast extract
  • .25% (w/v) tryptone

Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.

From Molecular Cloning