Difference between revisions of "GYT"

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(Ingredients)
 
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== Summary ==
 
== Summary ==
GYT Medium.  Used in preparing electrocompetent cells
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GYT Medium.  Used in preparing electrocompetent cells.
  
 
== Ingredients ==
 
== Ingredients ==
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*.25% (w/v) tryptone
 
*.25% (w/v) tryptone
  
Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Sore in 2.5-ml aliquots at 4°C.  
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Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.  
  
 
From ''Molecular Cloning''
 
From ''Molecular Cloning''

Latest revision as of 09:53, 22 July 2008

Summary

GYT Medium. Used in preparing electrocompetent cells.

Ingredients

  • 10% (v/v) glycerol
  • 0.125% (w/v) yeast extract
  • .25% (w/v) tryptone

Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Store in 2.5-ml aliquots at 4°C.

From Molecular Cloning