Difference between revisions of "GYT"

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(New page: == Summary == SOB Medium. Used in growing bacteria for preparing electrocompetent cells == Ingredients == *10% (v/v) glycerol *0.125% (w/v) yeast extract *.25% (w/v) tryptone Sterilize ...)
 
(Summary)
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== Summary ==
 
== Summary ==
SOB Medium.  Used in growing bacteria for preparing electrocompetent cells
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GYT Medium.  Used in preparing electrocompetent cells
  
 
== Ingredients ==
 
== Ingredients ==

Revision as of 09:00, 22 July 2008

Summary

GYT Medium. Used in preparing electrocompetent cells

Ingredients

  • 10% (v/v) glycerol
  • 0.125% (w/v) yeast extract
  • .25% (w/v) tryptone

Sterilize the medium by passing it through a prerinsed 0.22-μmfilter. Sore in 2.5-ml aliquots at 4°C.

From Molecular Cloning