Difference between revisions of "GFP"

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(GFP Variants)
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**This is GFPmut3b<cite>Cormack</cite> with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.<cite>Anderson</cite>
 
**This is GFPmut3b<cite>Cormack</cite> with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.<cite>Anderson</cite>
 
*[[Emerald]]
 
*[[Emerald]]
* EGFP F64L, S65T + optimized for human codons <cite>li97</cite>
+
* EGFP F64L, S65T + optimized for human codons with 35-fold increase in fluorescence over GFP <cite>li97</cite>
  
 
==References==
 
==References==

Revision as of 11:48, 27 September 2006

The fluorophore is tyrosine Y66 and the surrounding amino acids of Ser(65)-Tyr(66)-Gly(67) are also critical [1].

GFP Variants

  • GFPmut3b - S65G, S72A
  • GFPmut3* - mutations from wt: S2R,S65G,S72A
    • This is GFPmut3b[2] with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.[3]
  • Emerald
  • EGFP F64L, S65T + optimized for human codons with 35-fold increase in fluorescence over GFP [1]

References

  1. Li X, Zhang G, Ngo N, Zhao X, Kain SR, and Huang CC. Deletions of the Aequorea victoria green fluorescent protein define the minimal domain required for fluorescence. J Biol Chem. 1997 Nov 7;272(45):28545-9. PubMed ID:9353317 | HubMed [li97]
  2. Cormack BP, Valdivia RH, and Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 1996;173(1 Spec No):33-8. PubMed ID:8707053 | HubMed [Cormack]
  3. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, and Molin S. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol. 1998 Jun;64(6):2240-6. PubMed ID:9603842 | HubMed [Andersen]
All Medline abstracts: PubMed | HubMed

Molecular Biology and Muation of Green Fluorescent Protein (Book Chapter, Zacharias & Tsien)