Difference between revisions of "GFP"

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(GFP Variants)
(GFP Variants)
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==GFP Variants==
 
==GFP Variants==
 +
*GFPmut3b - S65G, S72A
 
*[http://parts.mit.edu/registry/index.php/Part:BBa_E0040 ''GFP''mut3*] - mutations from wt: S2R,S65G,S72A
 
*[http://parts.mit.edu/registry/index.php/Part:BBa_E0040 ''GFP''mut3*] - mutations from wt: S2R,S65G,S72A
**This is GFPmut3b<cite>Cormack</cite> with a degredation tag attached.
+
**This is GFPmut3b<cite>Cormack</cite> with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.
  
 
==References==
 
==References==

Revision as of 20:55, 30 May 2006

GFP Variants

  • GFPmut3b - S65G, S72A
  • GFPmut3* - mutations from wt: S2R,S65G,S72A
    • This is GFPmut3b[1] with a degredation tag attached + an accidental mutation at position 2 that doesn't affect function according to the authors.

References

  1. Cormack BP, Valdivia RH, and Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 1996;173(1 Spec No):33-8. PubMed ID:8707053 | HubMed [Cormack]

Molecular Biology and Muation of Green Fluorescent Protein (Book Chapter, Zacharias & Tsien)