Difference between revisions of "G418"

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m (Links: related antibiotics)
m (Links)
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* related antibiotics: [[kanamycin]], [[neomycin]]
* related antibiotics: [[kanamycin]], [[neomycin]] (all aminoglycosides)
* FAQ on G418 [http://www.g418.com/faq.htm]
* FAQ on G418 [http://www.g418.com/faq.htm]
* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]
* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]

Revision as of 05:20, 4 August 2006


  • stock solution: 200mg/mL (1000X), stored at -20 C
  • working concentration: working concentration depends on organism and purpose of antibiotic application; stable at RT
  • bacteria selection: 8-16 µg/ml
  • plant cell maintenance: 10 µg/ml
  • plant cell selection: 25-50 µg/ml
  • mammalian cell maintenance: 200 µg/ml
  • mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)
  • yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.


  • Gentamycin analog, used to select for the KanMX marker in yeast.
  • mode of action: irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
  • mode of resistance: neo/kan gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin and kanamycin by phosphorylation.


  • related antibiotics: kanamycin, neomycin (all aminoglycosides)
  • FAQ on G418 [1]
  • list of cell culture material by company [2]