Difference between revisions of "G418"

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(working concentrations by organism)
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===Working Concentration===
 
===Working Concentration===
After transformation incubate culture at 30 C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L G418.
+
Working concentration depends on organism and purpose of antibiotic application.
 +
 
 +
* bacteria selection: 8-16 µg/ml
 +
 
 +
* plant cell maintenance: 10 µg/ml
 +
* plant cell selection: 25-50 µg/ml
 +
 
 +
* mammalian cell maintenance: 200 µg/ml
 +
* mammalian cell selection: 400 µg/ml
 +
* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418.
 +
 
  
 
==Mode of Action==
 
==Mode of Action==
 +
 
Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.
 
Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.
  
 
==Safety==
 
==Safety==
 +
 +
==Links==
 +
* FAQ on G418 [http://www.g418.com/faq.htm]

Revision as of 04:47, 4 August 2006

Experimental

Gentamycin analog, used to select for the KanMX marker in yeast.

Stock Solution

200mg/mL (1000X), stored at -20 C

Working Concentration

Working concentration depends on organism and purpose of antibiotic application.

  • bacteria selection: 8-16 µg/ml
  • plant cell maintenance: 10 µg/ml
  • plant cell selection: 25-50 µg/ml
  • mammalian cell maintenance: 200 µg/ml
  • mammalian cell selection: 400 µg/ml
  • yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.


Mode of Action

Irreversibly binds to 80S ribosomal subunit, disrupting proofreading. Can be blocked by aminoglycoside-3'-phosphotransferase.

Safety

Links

  • FAQ on G418 [1]