G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/10/31

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Further Amplification of Discovered GFP and ATF1 Colonies

  • Today we retrieved the plates from the oven, upon which 10 collectable colonies were discovered growing. Luckily, these 10 colonies were spread out over three GFP plates and two ATF1 plates so that both desired genes had amplified. We labeled ten large tubes, pipetted 5mL of LB solution and 5ul of ampicillin - (to kill off any contaminating bacteria that might have sneaked into the plates and grown) - into the tubes, and collected samples from each colony. (This was done by swiping them with pipette tips and dropping the tips directly into the tubes.) The tubes were placed in the shaker overnight to continue propagation.