G.tigrina Hox gene DthoxC insertion into prokaryote E.coli / (by UNIamCloning)/2011/09/21

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DNA Extraction and Purification from G.tigrinia 9/21/11

  • DNA extraction using both halves of one planarian (Gt.1) was completed using the QIAGEN DNeasy blood and tissue kit. The kit calls for 20-200 µl of buffer AE. We used 35 µl Buffer AE on the assumption that our final yield of DNA would be low and to produce a higher concentration of DNA in the final sample.