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Version: 22 May 2007 Projects Magnetic bacteria: Jen Bell Magnetic bacteria: Dzianis Trubitsyn Conventional whole cell biosensors: Chris French 'Bacillosensors': Chris French and Lucy Montgomery Synthetic biology-based biosensors: Chris French, Xiaonan Wang, Nimisha Joshi Phytoremediation: Chris French Thiocyanate degradation: Chris French Flavin-dependent monooxygenases: Peter Wilson Bacteriohemerythrins: C. French Biofilms: Gary Dorken Making a broad host range BioBrick vector: Edinburgh iGEM2007 team and C. French Biobrick Protocols 1. Primer Design 2. Cloning parts by PCR with Pfu polymerase 2b. Cloning parts by PCR with Kod polymerase 3. Agarose Gel Electrophoresis 4. Purifying a PCR product from solution 5. Cloning a PCR product into a biobrick vector 6. Preparing and using competent E. coli cells 7. Screening colonies by PCR 8. Plasmid DNA minipreps 8b. Plasmid DNA maxipreps 9. Analytical restriction digests 10. Combining two biobricks to make a new biobrick 11. Adding a ribosome binding site to a biobrick coding sequence 12. BALTIC: a new method for combining standard BioBricks 13. MABEL: a fast easy method for removing unwanted restriction sites 14. BABEL: a new method for generating BioBricks without restriction digests. Other Protocols Growth of E. coli Growth of B. subtilis Transforming Bacillus subtilis Plasmid DNA minipreps Restriction digests Agarose gel electrophoresis PCR Purification of DNA fragments Ligation Cloning of PCR products Preparation of cell extracts Protein Assay SDS-PAGE Native-PAGE Enrichment and growth of environmental bacteria Identification of bacteria Gram stain Making glycerol stocks Assaying polysaccharides with the anthrone assay Lab information Catalog of primers Safety Information Hazardous chemicals list Other links C. French main page Bacteriology page SBS page University of Edinburgh.