Difference between revisions of "Frankel:Force Spectroscopy"

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<div style="padding: 10px; width: 800px; border: 5px solid white;">
 
  
'''<font color=#000000 font size=8>Force Spectroscopy</font>'''
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'''<font align="center" font color=#ffffff font size=8>______ </font>''''''<font align="center" font color=#000000 font size=8>Force Spectroscopy</font>'''
  
  
{| cellspacing="5px"
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{| cellspacing="3px"
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<div>
<div style="float:left;margin-left:0.3em;margin-right:0.7em">
 
  
'''<font color=#FFFFFF font size=8>HIV-gp160</font>'''
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|  align="center" width=400px style="border: 2px solid #000033; background-color:#FFFFFF; padding:1em;" class="plainlinks" valign="top"|
  
[[Image:Spectroscopy_complete.jpg|500px]]
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'''<font color=#000000 font size=3>Picking up and unfolding a single protein</font>'''
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[[Image:Spectroscopy_complete.jpg|350px]]
  
 
</div>
 
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| rowspan="2" width="70%" style="border: 2px solid #000033; background-color:#ffffff; padding:1em;" valign="top"|
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| align="center" rowspan="2" width=21px style="border: 2px solid #000033; background-color:#ffffff; padding:1em;" valign="top"|
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'''<font color=#FFFFFF font size=3>Assembly</font>'''
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'''<font color=#000000 font size=3>Nano-mechanics of HIV GP160</font>'''
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[[Image:GP160mica.png|300px]]
  
[[Image:Stlow.png|300px]]
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[[Image:Hislow.png|300px]]
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[[Image:GP_160.png|300px]]
[[Image:Sttr.png|300px]]
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[[Image:Histr.png|300px]]
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'''<font color=#FFFFFF font size=6>GP160mica</font>'''
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[[Image:Stlow.png|330px]]
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[[Image:Hislow.png|330px]]
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----
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'''<font color=#000000 font size=3 font align="justify">
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Typical sawtooth unfolding pattern of a single molecule of the HIV virion protein GP160. Unfolding forces can be extracted from the sawtooth pattern by applying the worm like chain model.</font>'''
  
  
 
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'''<font color=#000000 font size=3> Unfolding the ECM protein fibronectin </font>'''
  
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[[Image:FNsawt.png|300px]]
  
[[Image:FNsawt.png|300px]]
 
 
[[Image:HisFN.png|300px]]
 
[[Image:HisFN.png|300px]]
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----
 
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'''<font color=#000000 font size=3> Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface  was 85.1 mas menos 2.7 pN.
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'''<font align="justify" font size=3>  
</font>'''
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Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface  was 85.1 ± 2.7 pN.

Latest revision as of 17:22, 9 January 2013

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Home=Frankel Members=#,Principal Investigator=Frankel:Lab_Members, PhD students=Frankel:Lab_Members, Alumni=Frankel:Lab_Members Contact=Frankel:Contact Collaborators=Frankel:Collaborators Publications=Frankel:Publications Lab=Frankel:Research Research=#,Force Spectroscopy=Frankel:Force Spectroscopy,HIV/Virus=Frankel:HIV/Virus,ECM Proteins=Frankel:ECM Proteins,Cyberplasm=Frankel:Cyberplasm,Cancer=Frankel:Cancer


'______ 'Force Spectroscopy


Assembly

Nano-mechanics of HIV GP160


GP160mica.png


GP 160.png

GP160mica

Stlow.png

Hislow.png



Typical sawtooth unfolding pattern of a single molecule of the HIV virion protein GP160. Unfolding forces can be extracted from the sawtooth pattern by applying the worm like chain model.





Unfolding the ECM protein fibronectin

FNsawt.png

HisFN.png



Sawtooth pattern on the retraction force curve indicating the unfolding of fibronectin. The average rupture force distribution of the protein on mica surface was 85.1 ± 2.7 pN.