Fixing cells

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Formaldehyde Fixation

  • This works well for fixation of mammalian cells in preparation for immunolabeling and microscopy


-Fixation Solution: 4% Formaldehyde in PBS

-Permeabilization Solution: 0.1% Triton X-100 in PBS


1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.

2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.