Fixing cells

From OpenWetWare
Revision as of 22:12, 25 February 2006 by Bbelmont (talk | contribs)
Jump to: navigation, search

Formaldehyde Fixation

      • This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy

Reagents -Fixation Solution: 4% Formaldehyde in PBS -Permeabilization Solution: 0.1% Triton X-100 in PBS


1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.

2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.