Difference between revisions of "Fixing cells"

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'''Reagents'''
 
'''Reagents'''
 
-Fixation Solution: 4% Formaldehyde in PBS
 
-Fixation Solution: 4% Formaldehyde in PBS
 +
 
-Permeabilization Solution: 0.1% Triton X-100 in PBS
 
-Permeabilization Solution: 0.1% Triton X-100 in PBS
  

Revision as of 23:13, 25 February 2006

Formaldehyde Fixation

      • This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy

Reagents -Fixation Solution: 4% Formaldehyde in PBS

-Permeabilization Solution: 0.1% Triton X-100 in PBS

Protocol

1) For fixation, incubate cells in Formaldehyde Solution for 10-15 minutes at room temperature.

2) For permeabilization, remove Formaldehyde Solution, and incubate cells in Permeabilization Solution for 5 minutes at room temperature.