Difference between revisions of "Fixing cells"

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'''Formaldehyde Fixation'''
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== '''Formaldehyde Fixation''' ==
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***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
 
***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
  

Revision as of 23:11, 25 February 2006

Formaldehyde Fixation

      • This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy

1) Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.

2) For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature. There is no need to wash between fixation and permeabilization steps.