Difference between revisions of "Fixing cells"

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'''Formaldehyde Fixation'''
 
'''Formaldehyde Fixation'''
****This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy****
+
****This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy
  
 
1)  Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.
 
1)  Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.
  
 
2)  For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature.  There is no need for washing between fixation and permeabilization steps.
 
2)  For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature.  There is no need for washing between fixation and permeabilization steps.

Revision as of 22:03, 25 February 2006

Formaldehyde Fixation

        • This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy

1) Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.

2) For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature. There is no need for washing between fixation and permeabilization steps.