Difference between revisions of "Fixing cells"

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[[10x PBS]]
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'''Formaldehyde Fixation'''
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***This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy****
  
(Does this protocol want to treat upon mammalian cell fixation for microscopy? i.e. for HeLas, etc.? [[User:Levskaya|Levskaya]] 23:01, 7 February 2006 (EST))
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1)  Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.
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2) For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature. There is no need for washing between fixation and permeabilization steps.

Revision as of 22:02, 25 February 2006

Formaldehyde Fixation

      • This works well for fixation of mammalian cells in preparation for immunolabeleing and microscopy****

1) Incubate cells in ~4% Formaldehyde solution (in PBS) for 10-15 minutes at room temperature.

2) For permeabilization of cells, remove formaldehyde solution, and incubate cells in 0.1% Triton X-100 (in PBS) for 5 minutes at room temperature. There is no need for washing between fixation and permeabilization steps.