Difference between revisions of "Escherichia coli RNA polymerase"

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*0.5 mM NTPs
 
*0.5 mM NTPs
 
*1μg T7 D111 DNA as template
 
*1μg T7 D111 DNA as template
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 +
[[Category:Escherichia coli]] [[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:RNA]]

Latest revision as of 06:22, 24 May 2007

Epicentre is the only commercial source for Escherichia coli RNA polymerase.

Materials

Reaction conditions

For core enzyme

from Epicentre

For sigma saturated holoenzyme

from Epicentre

Functional test

50 μL reaction

  • 1X E. coli RNA polymerase transcription buffer
    • 0.04 M Tris-HCl (pH 7.5)
    • 0.15 M KCl
    • 10 mM MgCl2
    • 0.01% Triton® X-100
  • 2mM DTT
  • 0.25mM NTPs
  • 1μg T7 D111 DNA as template

Assay test

50 μL reaction

  • 1X E. coli RNA polymerase transcription buffer
    • 0.04 M Tris-HCl (pH 7.5)
    • 0.15 M KCl
    • 10 mM MgCl2
    • 0.01% Triton® X-100
  • 10 mM DTT
  • 0.5 mM NTPs
  • 1μg T7 D111 DNA as template