Difference between revisions of "Erickson:TAE Buffer"

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Line 5: Line 5:
 
''50X TAE Buffer''
 
''50X TAE Buffer''
 
   ''Tris-Acetate-EDTA''<br>
 
   ''Tris-Acetate-EDTA''<br>
   242 mg Tris Base
+
   242 g Tris Base
 
   57.1 mL Acetic Acid
 
   57.1 mL Acetic Acid
 
   100 mL  0.5M EDTA
 
   100 mL  0.5M EDTA
  
 
*Add ddH<sub>2</sub>O to 1 L and adjust pH to 8.5
 
*Add ddH<sub>2</sub>O to 1 L and adjust pH to 8.5

Latest revision as of 14:28, 30 November 2009


TAE Running Buffer

50X TAE Buffer

  Tris-Acetate-EDTA
242 g Tris Base 57.1 mL Acetic Acid 100 mL 0.5M EDTA
  • Add ddH2O to 1 L and adjust pH to 8.5