Ribosomes are supramolecular assembly of RNA and proteins, responsible for translating the genetic information. Ribosome consists of two unequal subunits (40S and 60S). The small subunit decodes the mRNA, whereas the large subunit catalyzes the formation of peptide bonds. The eukaryotic ribosome biogenesis is a complex multistep process where approximately 80 ribosomal proteins and 4 rRNAs are assembled into mature ribosomal particles (Kressler et al., 1999). More than 200 additional proteins and snoRNAs are needed for the biosynthesis of a eukaryotic ribosome.
The basic process of ribosome biogenesis is conserved within eukaryotes, but the most detailed results have been obtained with S.cerevisiae as the model system owing to its excellent genetic and biochemical suitability.
My lab´s primary interest is to understand the processing and assembly of ribosomal subunits in S.cerevisiae. We are presently working on the identification and significance of rRNA modifications in the 25S rRNA of Saccharomyces cerevisiae.
- Anatoli Lioutikov (Graduate Student)
- Britta Meyer (Post Doc.)
- Jun Yang (Graduate student)
- Peter Watzinger (Technical Assistant)
- Stefanie Lamberth (Technical Assistant)
- Sunny Sharma (Graduate Student)