Endy:dNTP stock solution

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Revision as of 11:52, 31 August 2005 by Josh K. Michener (talk | contribs)
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Each individual dNTP comes as 25 μmol in 100 mM solution. The lids are color-coded to each nucleic acid.

Protocol #1

  1. Dilute these in DI water to 2.5 mM to get four tubes of diluted dNTP, i.e., n*(1 μl dNTP + 39 μl water).
  2. Combine these in equal amounts to yield a 10x solution for use in PCR.

I don't know if there's any advantage other than to minimize freeze-thaw cycles to keeping intermediate dilutions of individual dNTPs rather than combining them immediately into the 10x solution.
Sean 17:50, 30 Aug 2005 (EDT)

Protocol #2

My dNTP stocks are:

  • 100x: 25mM each dNTP. Combine equal volumes of 100 mM individual dNTPs.
  • 10x: 2.5mM each dNTP. Dilute back 100x stock 1:10.
  • Working concentration: 250 μM

-Jkm 10:04, 31 Aug 2005 (EDT)