Endy:Victor3 plate variation

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Revision as of 14:37, 8 August 2005 by Barry Canton (talk | contribs)
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96 well plates with transparent bases cost ~$4 each. It was unclear if washing plates (bleach, followed by ethanol) led to greater variation in readings.


The GFP and absorbance of every well on a new and a washed plate were measured. The wells were empty.


Histograms of the GFP readings for each plate are shown below.

New plate
Old plate


  • The washed plate results in a higher average background reading.
  • The washed plate actually results in tighter distribution of readings.
  • For most applications, washed plates are perfectly acceptable.