Difference between revisions of "Endy:Victor3 plate variation"
|Line 10:||Line 10:|
Revision as of 14:37, 8 August 2005
96 well plates with transparent bases cost ~$4 each. It was unclear if washing plates (bleach, followed by ethanol) led to greater variation in readings.
The GFP and absorbance of every well on a new and a washed plate were measured. The wells were empty.
Histograms of the GFP readings for each plate are shown below.
- The washed plate results in a higher average background reading.
- The washed plate actually results in tighter distribution of readings.
- For most applications, washed plates are perfectly acceptable.