Difference between revisions of "Endy:Victor3 plate variation"

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==Background==
 
==Background==
96 well plates with transparent bases cost ~$4 each.  It was unclear if washing plates (bleach, followed by ethanol) led to greater variation in readings.
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96 well plates with transparent bases cost ~$4 each.  It would be desirable to reuse plates if possible. It was unclear if washing plates (bleach, followed by ethanol) led to unacceptable variation in readings.
  
 
==Method==
 
==Method==
The GFP and absorbance of every well on a new and a washed plate were measured.  The wells were empty.
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The GFP and absorbance at 600nm of every well on a new and a used plate that had been washed were measured.  The wells were empty.
  
 
==Results==
 
==Results==
Histograms of the GFP readings for each plate are shown below.
+
Histograms of the GFP readings for each plate are shown below.  Absorbance readings are not shown but showed similar trends to the GFP readings.
  
 
[[Image:NewplateGFP|thumb|300px|left|New plate]]
 
[[Image:NewplateGFP|thumb|300px|left|New plate]]
[[Image:OldplateGFP.jpg|thumb|300px|left|Old plate]]
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[[Image:OldplateGFP.jpg|thumb|300px|right|Old plate]]
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==Conclusions==
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*The washed plate has a higher average background reading.
 +
*The washed plate actually has a tighter distribution of readings.
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*For most applications, washed plates are perfectly acceptable.

Latest revision as of 13:45, 8 August 2005

Background

96 well plates with transparent bases cost ~$4 each. It would be desirable to reuse plates if possible. It was unclear if washing plates (bleach, followed by ethanol) led to unacceptable variation in readings.

Method

The GFP and absorbance at 600nm of every well on a new and a used plate that had been washed were measured. The wells were empty.

Results

Histograms of the GFP readings for each plate are shown below. Absorbance readings are not shown but showed similar trends to the GFP readings.

New plate
Old plate


Conclusions

  • The washed plate has a higher average background reading.
  • The washed plate actually has a tighter distribution of readings.
  • For most applications, washed plates are perfectly acceptable.