Difference between revisions of "Endy:Victor3 plate reader"

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(Choosing labels)
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[[Endy:Victor3 plate variation| Plate variation]]<br>
[[Endy:Victor3 plate variation| Plate variation]]<br>
[[Endy:Victor3 Anecdotal| Anecdotal]]<br>
[[Endy:Victor3 Anecdotal| Anecdotal]]<br>
[[Endy:Victor3 absorbance labels|Converting from absorbance to OD600]]

Revision as of 11:32, 5 January 2006


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The Perkin Elmer Victor3 Plate Reader is located in 68-558d. The manufacturer website is located here


Barry Canton is the point man in the Endy Lab if you would like to use this machine.

Getting Started

Overview of using the plate reader
Available filters


Creating a basic protocol

more here soon

Choosing labels

Detection limits and linear range

Setting an appropriate lamp energy

Running a protocol

Arranging samples

Counteracting evaporation in long time courses

Data Analysis

Basic data analysis
Measuring PoPS
Background measurements
Excel macro to hasten data processing
Variation in repeated measurements
Plate variation
Converting from absorbance to OD600

When things go bad

  • Instrument serial number - 4206012
  • Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
  • Endy:Victor3 Service History