Endy:PCR Supermix

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Revision as of 11:30, 23 July 2007 by Nishant M. Bhat (talk | contribs) (New page: For a 100µL reaction using the supermix: #100µL PCR Supermix #1.5µL forward primer (40 mM) #1.5µL reverse primer (40 mM) #0.5µL template DNA (10ng/µL) Protocol: #95 deg - 5 min #95...)
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For a 100µL reaction using the supermix:

  1. 100µL PCR Supermix
  2. 1.5µL forward primer (40 mM)
  3. 1.5µL reverse primer (40 mM)
  4. 0.5µL template DNA (10ng/µL)

Protocol:

  1. 95 deg - 5 min
  2. 95 deg - 30s
  3. 55 deg (or appropriate Tm) - 30s
  4. 68 deg - 1min/kbp of template
  5. Repeat steps 2-4 35x
  6. 68 deg - 10 min
  7. Store at 4 deg