Endy:NPE flow cytometer Calibration spheres

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Revision as of 06:40, 11 August 2005 by Jason R. Kelly (talk | contribs)
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We currently have spheres which can be used to:

  1. make sure the fluorescent channel(s) are behaving properly
  2. get a standard to correct day-to-day fluctuations in the readings of the fluorescent channels
  3. calibrate the electronic volume channel (EV) i.e. establish a correspondence between channel number and volume

The calibration we currently have (August 2005) were purchased from Spherotech. Other labs use spheres from Polysciences or Bangs Labs. Michael Brochu (Sr, I believe) said he uses 1um spheres with cat.#FS03F from Bangs Labs which give 7-8% CV (on the fluorenscence or the EV channel?]. I was unable to find that particular product on Bangs Labs website, though.