Difference between revisions of "Endy:Library-based construction"

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("Fixing" several broken inverters)
(Procedure)
 
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=="Fixing" several broken inverters==
 
=="Fixing" several broken inverters==
 
We will demonstrate the utility of the [[PoPS device screening plasmid|screening plasmid]] for fixing "broken" inverters via directed evolution.  Preliminary results with the tetR-based inverter <bbpart>Q04400</bbpart> showed early success of this method.
 
We will demonstrate the utility of the [[PoPS device screening plasmid|screening plasmid]] for fixing "broken" inverters via directed evolution.  Preliminary results with the tetR-based inverter <bbpart>Q04400</bbpart> showed early success of this method.
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==Procedure==
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[[Endy:Library-based construction/Inverter library protocol]]
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==Reshmaverters==
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We are working with Reshma Shetty to test her new inverter designs. 
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*[[Knight:Evolving Reshmaverters]] - project page.
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===Results===
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==TetR==
 
==TetR==
<add results here>
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===Results===
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Need to port the results described [[:Image:JK.SyntheticBiology2.0.Poster.pdf|here]].
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==Mnt==
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==penI==
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===Results===
  
 
==Contact==
 
==Contact==
 
*[[Kelly Chang]]
 
*[[Kelly Chang]]
 
*[[Jason Kelly]]
 
*[[Jason Kelly]]

Latest revision as of 10:49, 17 July 2006

This is a work in progress, bear with us.

"Fixing" several broken inverters

We will demonstrate the utility of the screening plasmid for fixing "broken" inverters via directed evolution. Preliminary results with the tetR-based inverter <bbpart>Q04400</bbpart> showed early success of this method.

Procedure

Endy:Library-based construction/Inverter library protocol

Reshmaverters

We are working with Reshma Shetty to test her new inverter designs.

Results

TetR

Results

Need to port the results described here.

Mnt

penI

Results

Contact