Here is a basic standard protocol that we try and use in the Endy and Knight labs. Exact conditions may depend on the strain, induction conditions etc.
When we are doing flow experiments, we normally use a FACScan cytometer, described here. Some lab members have experience using the MoFlo machines also. We also have an NPE flow cytometer that we use in our lab for some experiments.
We calibrate results from day to day using this bead protocol.